Bora Antibody

Q6PGQ7

Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Polyclonal

BORA

Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 µg/mL.

Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 µg/mL.

Q6PGQ7; 74737659

61203

C13orf34;

Bora antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Bora antibody can be used for detection of Bora by Western blot at 1-2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.

Bora Antibody is supplied in PBS containing 0.02% sodium azide.