N4BP1 Antibody

O75113

N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Polyclonal

N4BP1 (HGNC:29850)

Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 µg/mL.

Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 µg/mL.

NP_694574; 48928019

100379

N4BP1; At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

N4BP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.

N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.