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Vinculin Antibody [7E10]

Vinculin Antibody [7E10]

Item no.	ABC-ASC12026
Manufacturer	Abcepta
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, ELISA
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P18206
Application	WB, E
Bio Background	Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2, 3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Bio References	Burridge K, Fath K, Kelly T, et al. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol.1988; 4:487-525. Gilmore AP, Jackson P, Waites GT, et al. Further characterization of the talin-binding site in the cytoskeletal protein vinculin. J. Cell Sci. 1992; 103:719-31. Deakin NO, Ballestrem C, and Turner CE. Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA. PLoS One 2012; 7:e37990. Goldmann WH, Auernheimer V, Thievessen I, et al. Vinculin, cell mechanics and tumour cell invasion. Cell Biol. Int. 2013; Feb 1.
Clonality	Monoclonal
Gene ID	7414
Gene Name	VCL
Reactivity	H, M, Rat, Rb, C
Legend image 1	Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 µg/ml.
Type image 1	WB
Other Accession	NP_003364; 4507877
Isotype	IgG
Calculated MW	123799
Antigen Source	homo sapiens
Target/Specificity	VCL; Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Purification	Vinculin antibody is purified via Protein A prification.
Reconstitution & Storage	Vinculin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
Antibody Form	Liquid
Application Notes	Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 µg/ml.
Positive Control	PC-3 Cell Lysate;
Buffer	Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 10/2/2025

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