GAPDH Antibody [12D3D5] (biotin)

P04406

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Monoclonal

GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141}

Western blot analysis of GAPDH in multiple cell and tissue lysates with Biotin-GAPDH antibody at 1 µg/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, mouse liver, and rat liver, respectively.

7669492; NP_002037; 2597

36053

Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 µg/ml.