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HSP90 Antibody

HSP90 Antibody

Item no.	ABC-ASM10009-FITC-200
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	FITC
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P08238
Reactivity	H, M, Rat, Rb, C, F, Dog
Legend image 1	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x.
Type image 1	IHC
Dilution image 1	1:100
Legend image 2	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
Type image 2	IHC
Dilution image 2	1:100
Legend image 3	Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 3	WB
Legend image 4	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.
Type image 4	IHC
Dilution image 4	1:100
Legend image 5	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
Type image 5	IHC
Dilution image 5	1:100
Legend image 6	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Type image 6	IHC
Dilution image 6	1:100
Legend image 7	Western Blot analysis of Human HeLa cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.
Type image 7	WB
Dilution image 7	1:2500
Other Accession	NP_031381.2
Isotype	IgG2a
Target/Specificity	Detects 90kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Immunogen	Recombinant human HSP90beta
Purification	Protein G Purified
Legend image 8	Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ASM10009). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10009) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.
Dilution image 8	1:2500
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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