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GluN2A/NR2A Antibody

GluN2A/NR2A Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 594
Amount	Inquiry
Item no.	ABC-ASM10263-A594-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q00959
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (ASM10263). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (ASM10263) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN2A/NR2A Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (ASM10263). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (ASM10263) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_036705.3
Isotype	IgG2A
Target/Specificity	Detects ~170kDa. Does not react with NR2B.
Immunogen	Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10263-A594-100-datasheet.pdf

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Delivery expected until 7/4/2024