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Anti-Chicken IgG (H&L) (Fluorescein Conjugated) Secondary Antibody

Anti-Chicken IgG (H&L) (Fluorescein Conjugated) Secondary Antibody

Item no.	ABC-ASR1084
Manufacturer	Abcepta
Amount	500 ug
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Specific against	other
Conjugate/Tag	FITC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Bio Background	Anti-Chicken IgG/IgY FITC conjugated secondary antibody is ideal for FLOW Cytometry and Immunofluorescence Microscopy.
Clonality	Polyclonal
Subtitle	Polyclonal, Fluorescein (FITC)
Legend image 1	Western Blot of Anti-Chicken IgG (H&L) (RABBIT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule . Lane 2: Chicken IgG F(c) Fragment . Lane 3: Chicken IgG F(ab) Fragment . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody 1:3, 000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40, 000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.
Type image 1	WB
Immunogen	Chicken IgG whole molecule
Concentration (mg/ml)	1.0 mg/mL by UV absorbance at 280 nm
Conjugate	Fluorescein (FITC)
Application Note	FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value	3.0 moles Fluorescein (FITC) per mole of IgG
Target Species	Chicken
Physical State	Lyophilized
Target Isotype	IgG (H&L)
Host Isotype	IgG
Reconstitution Volume	500 µL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide
Shipping Condition	Ambient
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASR1084-datasheet.pdf

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Amount: 500 ug

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Delivery expected until 9/4/2025

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