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Anti-Mouse IgG3 (Biotin Conjugated) Pre-adsorbed Secondary Antibody

Anti-Mouse IgG3 (Biotin Conjugated) Pre-adsorbed Secondary Antibody

Item no.	ABC-ASR2728
Manufacturer	Abcepta
Amount	1 mg
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Specific against	other
Host	Goat
Conjugate/Tag	Biotin
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Bio Background	Mouse IgG3 secondary antibody is available in a variety of formats. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Clonality	Polyclonal
Subtitle	Goat Polyclonal, Biotin
Reactivity	M
Immunogen	Mouse IgG3 heavy chain
Concentration (mg/ml)	1.0 mg/mL by UV absorbance at 280 nm
Conjugate	Biotin
Application Note	ELISA 1:20, 000-1:100, 000; Western Blot 1:2, 000-1:10, 000; Immunochemistry 1:1, 000-1:5, 000
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species	Mouse
Physical State	Lyophilized
Target Isotype	IgG3
Host Isotype	IgG
Reconstitution Volume	1.0 mL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide
Shipping Condition	Ambient
Purity	Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition	Store second antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 mg

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Delivery expected until 10/2/2025

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