Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

Anti-Rabbit IgG (H&L) antibody generated in donkey detects specifically rabbit IgG (H&L). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Donkey Polyclonal, Alkaline Phosphatase (Calf Intestine)

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) 1:7, 500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody 1:40, 000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

1:1, 000 - 1:5, 000

1.0 mg/mL by UV absorbance at 280 nm

ELISA 1:4, 000-1:16, 000; Western Blot 1:1, 000-1:5, 000; Immunochemistry 1:400-1:2, 000

Rabbit

IgG (H&L)

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Wet Ice

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.