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ARF5 Antibody (monoclonal) (M01)

ARF5 Antibody (monoclonal) (M01)

Item no.	ABC-AT1176a
Manufacturer	Abcepta
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Applications	WB, IF, ELISA
Clone	1B4
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ARF5, ADP-ribosylation factor 5
Available
Primary Accession	P84085
Antigen Type	Recombinant Protein
Application	WB, IF, E
Bio Background	ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.
Bio References	1.A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.Reiling JH, Olive AJ, Sanyal S, Carette JE, Brummelkamp TR, Ploegh HL, Starnbach MN, Sabatini DMNat Cell Biol. 2013 Dec; 15(12):1473-85. doi: 10.1038/ncb2865. Epub 2013 Nov 3.2.ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HWMol Biol Cell. 2013 Aug; 24(16):2570-81. doi: 10.1091/mbc.E13-04-0197. Epub 2013 Jun 19.3.GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis.Takashima K, Saitoh A, Hirose S, Nakai W, Kondo Y, Takasu Y, Kakeya H, Shin HW, Nakayama K.Cell Struct Funct. 2011; 36(2):223-35.4.Class II ADP-Ribosylation factors are required for efficient secretion of dengue viruses.Kudelko M, Brault JB, Kwok K, Li MY, Pardigon N, Peiris JS, Bruzzone R, Despres P, Nal B, Wang PG.J Biol Chem. 2011 Nov 21.5.Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity.Puxeddu E, Uhart M, Li CC, Ahmad F, Pacheco-Rodriguez G, Manganiello VC, Moss J, Vaughan M.Proc Natl Acad Sci U S A. 2009 Apr 14; 106(15):6158-63. Epub 2009 Mar 30.6.EFA6 facilitates the assembly of the tight junction by coordinating an Arf6-dependent and independent pathway.Klein S, Partisani M, Franco M, Luton F.J Biol Chem. 2008 Oct 31; 283(44):30129-38. Epub 2008 Sep 8.7.The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles.Islam A, Shen X, Hiroi T, Moss J, Vaughan M, Levine SJ.J Biol Chem. 2007 Mar 30; 282(13):9591-9. Epub 2007 Feb 2.
Clonality	Monoclonal
Gene ID	381
Gene Name	ARF5
Subtitle	Mouse monoclonal antibody raised against a partial recombinant ARF5.
Reactivity	H, M, Rat
Legend image 1	Immunofluorescence of monoclonal antibody to ARF5 on HeLa cell. [antibody concentration 15 ug/ml]
Type image 1	IF
Legend image 2	Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
Type image 2	WB
Dilution image 2	1:500~1000
Legend image 3	ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in HeLa ( (Cat # AT1176a )
Type image 3	WB
Dilution image 3	1:500~1000
Legend image 4	ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in PC-12((Cat # AT1176a )
Type image 4	WB
Dilution image 4	1:500~1000
Legend image 5	ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in Raw 264.7((Cat # AT1176a )
Type image 5	WB
Dilution image 5	1:500~1000
Legend image 6	ARF5 monoclonal antibody (M01), clone 1B4 Western Blot analysis of ARF5 expression in A-431 ( (Cat # AT1176a )
Type image 6	WB
Dilution image 6	1:500~1000
Legend image 7	Western Blot analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4. Lane 1: ARF5 transfected lysate(20.5 KDa). Lane 2: Non-transfected lysate.
Type image 7	WB
Dilution image 7	1:500~1000
Other Accession	BC003043
Isotype	IgG1 Kappa
Antigen Source	Human: ~~ Positive
Target/Specificity	ARF5 (AAH03043, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
NCBI Accession	NP_001653.1
	Clone: 1B4
Legend image 8	Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.
Org Accession	AAH03043
Gene Description	ADP-ribosylation factor 5
Gene Summary	ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 9/4/2025

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