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HDAC1 Antibody (monoclonal) (M14)

HDAC1 Antibody (monoclonal) (M14)

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Clone	5C11
Format	Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Applications	WB, IF, IHC, ELISA
Amount	100 ug
Host	Mouse
Item no.	ABC-AT2337a
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q13547
Antigen Type	Recombinant Protein
Application	WB, IHC, IF, E
Bio Background	Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Bio References	Combination of polymorphisms within the HDAC1 and HDAC3 gene predict tumor recurrence in hepatocellular carcinoma patients that have undergone transplant therapy. Yang Z, et al. Clin Chem Lab Med, 2010 Aug 24. PMID 20731616.CCDC6 represses CREB1 activity by recruiting histone deacetylase 1 and protein phosphatase 1. Leone V, et al. Oncogene, 2010 Jul 29. PMID 20498639.Regulation of Nur77 protein turnover through acetylation and deacetylation induced by p300 and HDAC1. Kang SA, et al. Biochem Pharmacol, 2010 Sep 15. PMID 20438716.Class I histone deacetylases 1, 2 and 3 are highly expressed in classical Hodgkin's lymphoma. Adams H, et al. Expert Opin Ther Targets, 2010 Jun. PMID 20415600.Double PHD fingers protein DPF2 recognizes acetylated histones and suppresses the function of estrogen-related receptor alpha through histone deacetylase 1. Matsuyama R, et al. J Biol Chem, 2010 Jun 11. PMID 20400511.
Clonality	Monoclonal
Gene ID	3065
Gene Name	HDAC1 (HGNC:4852)
Subtitle	Mouse monoclonal antibody raised against a full length recombinant HDAC1.
Reactivity	H, M, Rat
Legend image 1	Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml]
Type image 1	IF
Legend image 2	Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (78.76 KDa) .
Type image 2	WB
Dilution image 2	1:500~1000
Legend image 3	HDAC1 monoclonal antibody (M14), clone 5C11. Western Blot analysis of HDAC1 expression in PC-12 ( (Cat # AT2337a )
Type image 3	WB
Dilution image 3	1:500~1000
Legend image 4	HDAC1 monoclonal antibody (M14), clone 5C11 Western Blot analysis of HDAC1 expression in Hela S3 NE ( (Cat # AT2337a )
Type image 4	WB
Dilution image 4	1:500~1000
Legend image 5	HDAC1 monoclonal antibody (M14), clone 5C11. Western Blot analysis of HDAC1 expression in Raw 264.7 ( (Cat # AT2337a )
Type image 5	WB
Dilution image 5	1:500~1000
Legend image 6	HDAC1 monoclonal antibody (M14), clone 5C11. Western Blot analysis of HDAC1 expression in NIH/3T3 ( (Cat # AT2337a )
Type image 6	WB
Dilution image 6	1:500~1000
Legend image 7	Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysate(55.1 KDa). Lane 2: Non-transfected lysate.
Type image 7	WB
Dilution image 7	1:500~1000
Other Accession	BC000301
Isotype	IgG1 Kappa
Antigen Source	Human: ~~ Positive
Target/Specificity	HDAC1 (AAH00301, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
NCBI Accession	NP_004955.2
	Clone: 5C11
Legend image 8	Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Legend image 9	Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.
Type image 9	E
Legend image 10	Proximity Ligation Analysis of protein-protein interactions between NFKB1 and HDAC1 HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Org Accession	AAH00301
Gene Description	histone deacetylase 1
Gene Summary	Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq

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Amount: 100 ug

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Delivery expected until 7/4/2024