EEF1E1 Antibody

O43324

IF, FC, WB

Motegi H., et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Mao M., et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Kalnine N., et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mungall A.J., et al.Nature 425:805-811(2003).
Bienvenut W.V., et al.Submitted (DEC-2008) to UniProtKB.

9521

Purified Mouse Monoclonal Antibody (Mab)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AW5638 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

1:25

WB

Overlay histogram showing Hela cells stained with AW5638 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5638, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

19811

HUMAN