Epidermal Growth Factor (EGF) (**Biotin Labeled)

Biotin-Conjugated Mouse anti-Human EGF Monoclonal Antibody (Tracer)

Purified recombinant human EGF, 6kDa, 53 amino acid residues

Protein G affinity purified

No cross-reactivity with other human cytokines such as IL-1beta, TGF-beta1, IL-8, MCP-1, and SAA.

Epidermal growth factor (EGF), along with its receptor EGFR, plays an important role in cell proliferation, differentiation and survival. The binding of EGF to EGFR triggers the intrinsic tyrosine kinase activity of EGFR, activates signalling molecules and stimulates the synthesis of proteins and DNAs.
EGF is crucial for maintaining mucosal lining in gastrointestinal duct and for healing of oral and gastroesophageal ulcers. EGF signalling is needed for cancer growth. Monoclonal antibodies that block EGFR have been applied for lung cancer and colon inhibition.

ELISA: This Biotin-conjugated monoclonal antibody can be used as a tracer/detection antibody in a sandwich ELISA for human EGF detection in combination with a capture antibody (Cat. No.: MO-C40044D). Suggested Capture Coating Dose: 0.3ug/well; Substrate: TMB. If the suggested conditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL of EGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.

Store at -20C