Anti-C5 Antibody [046-10.2.2 (10-04)]

Mouse monoclonal [046-10.2.2 (10-04)] antibody to C5.

Purified human C5.

Supplied in Borate Buffered Saline with 0.1% Sodium Azide.

This antibody recognises secreted complement component 5 (C5), a ~190 kDa serine protease present in blood serum. It is composed of an ~115 kDa disulphide linked alpha-chain and a ~75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL-10 and IL-12 production.C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex. Mouse anti Human C5 antibody, clone 046-10.2.2 blocks C5 haemolytic activity. Removal of sodium azide is recommended prior to use in functional assays.