Anti-Thymosin Beta 4 (Tb4)

The predominant form of thymosin, thymosin b4, is a member of a highly conserved family of actin monomer-sequestering proteins. b-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin. Thymosin b4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin b4 is regarded as the principal actin-sequestering protein. Tb4 binds ATP G- monomeric actin in a 1:1 complex where G-actin cannot polymerize.

Western blotting: 1:100-400, Immunocytochemistry in formalin fixed cells: 1:100-500, Immunohistochemistry in formalin fixed frozen section: 1:100-500, Immunohistochemistry in paraffin section: 1:50-417

TMSB4X, FX, PTMB4, TB4X, TMSB4, Thymosin, Beta 4, X-Linked

Rat