Anti-IgG IgA IgM (Fc specific), Biotin conjugate

The reactivity of the antiserum is directed to the Fc subunit of the major immunoglobulin isotypes which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of human origin in the middle layer of the indirect test procedure, in nonisotopic assay methodology (e.g. ELISA) to measure immunoglobulins in human serum or other body fluids. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. As a second step an avidin or streptavidin conjugate of the user's choice has to be used.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1, 000 and 1:10, 000.

Biotin-conjugated IgG fraction of polyclonal Goat anti-Serum to Human IgG, IgA and IgM, Fc specific

Purified normal IgG, IgA and IgM containing fractions isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

For Research Use Only.