Anti-IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) (non Human), Biotin conjugate

The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In immunocytochemical and immunohistochemical staining of monoclonal mouse monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1, 000 and 1:10, 000.

Biotin-conjugated IgG fraction of polyclonal Rabbit anti-Serum to Mouse IgG, IgA and IgM, heavy and light chains, adsorbed for Human cross-reactivity.

Purified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

For Research Use Only.