Anti-CD11a (inhibitor)

Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide), also known as ITGAL, is a Human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common beta-chains (CD18). LFA-1 also has a distinct alpha-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes, it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstRated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to prolifeRate. The antibody was selected for inhibition of T-cell mediated killing.

The monoclonal antibody SPV-L7 reacts with the alpha chain of LFA-1, the CD11a/CD18 integrin heterodimer present on Human T- and B-lymphocytes, granulocytes and monocytes.

This antibody was obtained by immunisation of Balb/c mice with cells of the T4+T8- cytotoxic T lymphocyte clone HG-38. This antibody was selected for inhibition of T-cell mediated killing by screening of the hybridoma supernatants 10-14 days after the fusion.

For Research Use Only.