Comparison

CD100 (Semaphorin-4D)(A8), 1mg/mL

Item no. B-BNUM0218-50
Manufacturer Biotium
Amount 50 uL
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, ELISA
Clone A8
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Monkey (Cynomolgus, Simian)
Host Mouse
Isotype IgG1 Kappa
Citations PNAS USA (1996) 93:11780-11785. (Flow)PLoS One (2007) 2(9):e818. (Flow, WB, ELISA)
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias CD100,Semaphorin-4D,SEMA4D
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary
Manufacturer - Applications
Flow, surface (published), ELISA (published), WB (published)
Manufacturer - Category
Primary Antibodies Only
Manufacturer - Targets
CD100|Semaphorin-4D
Manufacturer - Conjugate / Tag
Purified, BSA-free
Manufacturer - Host
Mus musculus (mouse)
Shipping Temperature
Room Temperature (Next day)
Storage Conditions
Store at -10 to -35 °C|Protect fluorescent conjugates from light
Molecular Weight
50 kDa (Monomer)
Manufacturer - Research Area
Immunology|Neuroscience|Signal transduction
Description
CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse)
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration
1 mg/mL
Storage buffer
PBS, no BSA, no azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
PHA stimulated human peripheral blood lymphocytes.
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
SEMA4D
Unigene
494406
Positive Control
Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.
Antibody target cellular localization
Plasma membrane
Cell tissue expression
Hematopoietic cells
Antibody applications
Flow, surface (published)|ELISA (published)|WB (published)
Antibody application notes
Functional studies: Order Ab without azide; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.
Expected Antibody Applications
ELISA (published for clone), Flow, surface (published for clone), WB (published for clone)
Manufacturer - Antibody Reactivity
CD100|Semaphorin-4D
Antibody number
#0218

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
available

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