Comparison

Milk Fat Globulin(MFG-06), 1mg/mL

Item no. B-BNUM0227-50
Manufacturer Biotium
Amount 50 uL
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, IF, IHC
Clone MFG-06
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Citations Mol Endocrinol (2010) 24(2): 359-369. (WB)Lab Invest (2014) 94:1260-1272. (IHC, FFPE; IF)Exp Cell Res (2020) 392(1): 112013. (IF)
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Breast epithelial antigen BA46,EDIL1,HMFG,Lactadherin,Lactadherin precursor,Medin,MFGE8,MFGM,Milk fat globule-EGF factor 8 (MFG-E8)
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary
Manufacturer - Applications
IF (published), IHC, FFPE (verified), WB (published)
Manufacturer - Category
Primary Antibodies Only
Manufacturer - Targets
Milk-Fat-Globulin
Manufacturer - Conjugate / Tag
Purified, BSA-free
Manufacturer - Host
Mus musculus (mouse)
Shipping Temperature
Room Temperature (Next day)
Storage Conditions
Store at -10 to -35 °C|Protect fluorescent conjugates from light
Molecular Weight
45 kDa
Description
Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse)
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration
1 mg/mL
Storage buffer
PBS, no BSA, no azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Human milk fat globule membrane preparation
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
MFGE8
Unigene
3745
Positive Control
Normal breast or breast carcinoma
Antibody target cellular localization
Plasma membrane
Cell tissue expression
Epithelial cells|Mammary gland
Antibody applications
IF (published)|IHC, FFPE (verified)|WB (published)
Verified antibody applications
IHC (FFPE) (verified)
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Expected Antibody Applications
IF (published for clone), WB (published for clone)
Manufacturer - Antibody Reactivity
Milk-Fat-Globulin
Antibody number
#0227

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
available

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