Ep-CAM / CD326(323/A3), 1mg/mL

Primary

Primary Antibodies Only

Purified, BSA-free

Room Temperature (Next day)

40-43 kDa

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse)

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodologyimmunostaining detection application

1 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

TACSTD1

HT29 cells or breast tumor

Epithelial cells

Flow (verified)|IHC, FFPE (verified)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with rat; others not known|Immunohistology formalin-fixed 0.5-1.0 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

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