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CD100(133-1C6), 1mg/mL

CD100(133-1C6), 1mg/mL

Item no.	B-BNUM0932-50
Manufacturer	Biotium
Amount	50 uL
Category	Oncology Neuroscience Neural Adhesion Molecules Antibodies By Cell Type Flow Cytometry ELISA Primary Antibodies Immunostaining
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, ELISA
Clone	133-1C6
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgM Kappa
Citations	Frontiers Immunol (2017) 8: 765. (functional studies)Frontiers Immunol (2021) 12:240. (ELISA)Paterson J., Ailles L.E. (2018) High Throughput Flow for Cell Surface Profiling. In: Hawley T., Hawley R. (eds) Flow Protocols. Methods in Molecular Biology, vol 1678. Humana Press, New York, NY. (Flow)
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	CD100,Semaphorin-4D,SEMA4D
Shipping Condition	Room temperature
Available
Specificity	Baboon, Human
Manufacturer - Type	Primary
Manufacturer - Applications	Flow, surface (published), Functional studies (published), ELISA (published)
Manufacturer - Category	Primary Antibodies Only
Manufacturer - Targets	CD100\|Semaphorin-4D
Manufacturer - Conjugate / Tag	Purified, BSA-free
Manufacturer - Host	Mus musculus (mouse)
Shipping Temperature	Room Temperature (Next day)
Storage Conditions	Store at -10 to -35 °C\|Protect fluorescent conjugates from light
Molecular Weight	50 kDa (Monomer)
Manufacturer - Research Area	Immunology\|Neuroscience\|Signal transduction
Description	CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.
Product origin	Animal
Ingredient of biological origin	Mus musculus (mouse)
Undated stability guarantee in PI	2 years
Stability at RT during shipping (protected from light)	Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity	41116161
UNSPSC Commodity Title	Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals	PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration	1 mg/mL
Storage buffer	PBS, no BSA, no azide
Dated shelf life printed on label	Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)	PHA stimulated human peripheral blood lymphocytes.
Clonality	Monoclonal
Regulatory Status	For research use only (RUO)
Human Gene Symbol	SEMA4D
Unigene	494406
Positive Control	Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.
Antibody target cellular localization	Plasma membrane
Cell tissue expression	Hematopoietic cells
Antibody applications	Flow, surface (published)\|Functional studies (published)\|ELISA (published)
Antibody application notes	Immunofluorescence: 0.5-1 ug/mL\|Does not react with horse, others not tested\|Flow Cytometry 0.5-1 ug/million cells/0.1 mL\|Optimal dilution for a specific application should be determined by user
Expected Antibody Applications	ELISA (published for clone), Flow, surface (published for clone), Functional studies (published for clone)
Manufacturer - Antibody Reactivity	CD100\|Semaphorin-4D
Antibody number	#0932

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 11/27/2025

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