Comparison

Smooth Muscle Actin(ACTA2/791 +1A4), 1mg/mL

Item no. B-BNUM1203-50
Manufacturer Biotium
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications IF, IHC
Clone ACTA2/791 + 1A4
Specific against Human (Homo sapiens), Rat (Rattus norvegicus)
Host Mouse
Isotype IgG
Conjugate/Tag BSA
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary
Manufacturer - Applications
IF (verified)|IHC, FFPE (verified)
Manufacturer - Category
Antibodies
Manufacturer - Isotype
IgG' s
Manufacturer - Targets
Smooth Muscle Actin
Manufacturer - Conjugate / Tag
Purified, BSA-free
Manufacturer - Host
Mus musculus (mouse)
Shipping Temperature
Room temperature
Storage Conditions
Store at -10 to -35 °C|Protect fluorescent conjugates from light
Molecular Weight
42 kDa
Manufacturer - Research Area
Cytoskeleton
Description
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse)
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration
1 mg/mL
Storage buffer
PBS, no BSA, no azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791)
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
ACTA2
Unigene
500483
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Antibody target cellular localization
Cytoskeleton
Cell tissue expression
Smooth muscle
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Predicted to show broad reactivity|Optimal dilution for a specific application should be determined by user
Manufacturer - Synonym
ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46
Manufacturer - Antibody Reactivity
Smooth Muscle Actin

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
available

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