Clathrin, Heavy Chain(CHC/1432), 1mg/mL

IF (verified)

Clathrin, Heavy Chain

Mus musculus (mouse)

Store at -10 to -35 °C|Protect fluorescent conjugates from light

Recognizes protein of 192 kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse)

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodologyimmunostaining detection application

1 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

CLTC

HeLa cells. Placenta or Prostate Carcinoma.

IF (verified)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.

#1432