Comparison

S100A4 (Marker of Tumor Metastasis) (S100A4/1481), 1mg/mL

Item no. B-BNUM1481-50
Manufacturer Biotium
Amount 50 uL
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IHC
Clone S100A4/1481
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Mouse
Isotype IgG1 Kappa
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias 18A2,42A,CAPL,FSP1,Malignant transformation suppression 1 (MTS1),Metastasin,P9KA,PEL98,Placental calcium-binding protein,S100 calcium-binding protein A4,S100A4
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary
Manufacturer - Applications
Flow, intracellular (verified), IF (verified), IHC, FFPE (verified), WB (verified)
Manufacturer - Category
Primary Antibodies Only
Manufacturer - Targets
S100A4
Manufacturer - Conjugate / Tag
Purified, BSA-free
Manufacturer - Host
Mus musculus (mouse)
Shipping Temperature
Room Temperature (Next day)
Storage Conditions
Store at -10 to -35 °C|Protect fluorescent conjugates from light
Molecular Weight
10-12 kDa
Manufacturer - Research Area
Cancer
Description
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, alpha-6/beta-4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse)
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration
1 mg/mL
Storage buffer
PBS, no BSA, no azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Recombinant human S100A4 protein
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
S100A4
Unigene
654444
Positive Control
HeLa, A549 or A375 Cells. Placenta
Antibody target cellular localization
Nucleus & cytoplasm
Validated in protein array
Monospecific
Antibody applications
Flow, intracellular (verified)|IF (verified)|IHC, FFPE (verified)|WB (verified)
Verified antibody applications
Flow (intracellular) (verified)|IF (verified)|IHC (FFPE) (verified)|WB (verified)
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin): 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
S100A4
Antibody number
#1481

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
available

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