Comparison

Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) (KRTH/1576R), 1mg/mL

Item no. B-BNUM1576-50
Manufacturer Biotium
Amount 50 uL
Category
Type Antibody Primary
Format Liquid
Applications IHC
Clone KRTH/1576R
Specific against Human (Homo sapiens)
Host Rabbit
Isotype IgG
Conjugate/Tag BSA
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary
Manufacturer - Applications
IHC, FFPE (verified)
Manufacturer - Category
Antibodies
Manufacturer - Targets
Cytokeratin, Basic
Manufacturer - Conjugate / Tag
Purified, BSA-free
Manufacturer - Host
Oryctolagus cuniculus (domestic rabbit)
Shipping Temperature
Room temperature
Storage Conditions
Store at -10 to -35 °C|Protect fluorescent conjugates from light
Molecular Weight
52-67 kDa
Manufacturer - Research Area
Cancer|Cytoskeleton
Description
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Oryctolagus cuniculus (domestic rabbit)
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration
1 mg/mL
Storage buffer
PBS, no BSA, no azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Recombinant full-length human KRT76 protein
Clonality
Recombinant Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
KRT76
Unigene
654392
Positive Control
Epithelial cells, Skin or Adenocarcinomas
Antibody target cellular localization
Cytoskeleton
Cell tissue expression
Epidermal cells|Epithelial cells
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.25-0.5 ug/mL|Immunofluorescence 1-2 ug/mL Western blotting 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
Cytokeratin, Basic

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 uL
Available: In stock
available

Delivery expected until 9/25/2025 

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close