MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) (rMUC18/1130), 1mg/mL

Primary

Antibodies

Purified, BSA-free

Room temperature

130 kDa

Mel-CAM belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse)

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodology
immunostaining detection application

1 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Recombinant Monoclonal

MCAM

A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1.0 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 8.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

CD146|MCAM|MUC18