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Anti-DNA Ligase III/LIG3 Antibody Picoband®

Anti-DNA Ligase III/LIG3 Antibody Picoband®

Item no.	BOS-A02741-2
Manufacturer	Boster
Amount	100 ug/vial
Category	Antibodies Primary Antibodies
Type	Antibody
Format	Lyophilized
Applications	WB, FC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Citations	1. Chen, J., Tomkinson, A. E., Ramos, W., Mackey, Z. B., Danehower, S., Walter, C. A., Schultz, R. A., Besterman, J. M., Husain, I. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination. Molec. Cell. Biol. 15: 5412-5422, 1995. 2. Gao, Y., Katyal, S., Lee, Y., Zhao, J., Rehg, J. E., Russell, H. R., McKinnon, P. J. DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair. Nature 471: 240-244, 2011. 3. Moser, J., Kool, H., Giakzidis, I., Caldecott, K., Mullenders, L. H. F., Fousteri, M. I. Sealing of chromosomal DNA nicks during nucleotide excision repair requires XRCC1 and DNA ligase III-alpha in a cell-cycle-specific manner. Molec. Cell 27: 311-323, 2007.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Platelet basic protein; PBP; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; PPBP; CTAP3; CXCL7; SCYB7; TGB1; THBGB1; NAP-2
Available
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Observed Molecular Weight	113 kDa
Calculated Molecular weight	12252 MW
Clonality	Polyclonal
Application Details	Western blot, 0.25-0.5 ?g/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 ?g/1x10⁶ cells, Human ELISA, 0.1-0.5 ?g/ml, -
Manufacturer - Gene Name	LIG3
Gene Full Name	Neutrophil-activating peptide 2
Background	DNA ligase 3 is an enzyme that, in humans, is encoded by the LIG3 gene. This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Immunogen	E.coli-derived human DNA Ligase III/LIG3 recombinant protein (Position: D297-D963).
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets
Protein Function	LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2 (73), NAP-2 (74), NAP-2 (1-66), and most potent NAP-2 (1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III (1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
Description	Boster Bio Anti-DNA Ligase III/LIG3 Antibody Picoband® catalog # A02741-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A02741-2-datasheet.pdf

BOS-A02741-2-safety-datasheet.pdf