Comparison

Anti-Rad9/Rad9a Antibody Picoband® European Partner

Item no. BOS-A04161-1
Manufacturer Boster
Amount 100 ug/vial
Category
Type Antibody
Format Lyophilized
Applications WB, FC, IHC, ELISA
Specific against Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Sensitivity <10pg/ml
Citations 1. Lieberman, H. B., Hopkins, K. M., Nass, M., Demetrick, D., Davey, S. A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene. Proc. Nat. Acad. Sci. 93: 13890-13895, 1996.
2. Maniwa, Y., Yoshimura, M., Bermudez, V. P., Yuki, T., Okada, K., Kanomata, N., Ohbayashi, C., Hayashi, Y., Hurwitz, J., Okita, Y. Accumulation of hRad9 protein in the nuclei of nonsmall cell lung carcinoma cells. Cancer 103: 126-132, 2005.
3. Volkmer, E., Karnitz, L. M. Human homologs of Schizosaccharomyces pombe Rad1, Hus1, and Rad9 form a DNA damage-responsive protein complex. J. Biol. Chem. 274: 567-570, 1999.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Erf1; Protein Cl1; TB3-1; ETF1; ERF1; RF1; SUP45L1
Available
Manufacturer - Category
Primary Antibodies
Manufacturer - Isotype
Rabbit IgG
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
55-60 kDa
Calculated Molecular weight
90314 MW
Clonality
Polyclonal
Application Details
Western blot, 0.25-0.5?g/ml, Mouse, Rat

Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Mouse, Rat

Flow Cytometry (Fixed), 1-3?g/1x106 cells, Mouse

ELISA, 0.1-0.5?g/ml, -
Manufacturer - Gene Name
Rad9a
Gene Full Name
eukaryotic translation termination factor 1
Background
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen
E.coli-derived mouse Rad9/Rad9a recombinant protein (Position: M1-G389).
Contents
Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category
Adapters, Cell Type Marker, Intracellular Signaling, Neuron Marker, Neuroscience, Neurotransmission, Synapse Marker
Protein Name
Chromodomain-helicase-DNA-binding protein 2
Protein Function
s the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.
Subcellular Localization
Cytoplasm.
Description
Boster Bio Anti-Rad9/Rad9a Antibody Picoband® catalog # A04161-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug/vial
Available: In stock
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