Comparison

Anti-GLIS1 Antibody Picoband® European Partner

Item no. BOS-A12698-1
Manufacturer Boster
Amount 100 ug/vial
Category
Type Antibody
Format Lyophilized
Applications WB, FC, IF, IHC, ICC, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
Isotype IgG
Citations 1. Kim, Y.-S., Lewandoski, M., Perantoni, A. O., Kurebayashi, S., Nakanishi, G., Jetten, A. M. Identification of Glis1, a novel Gli-related, Kruppel-like zinc finger protein containing transactivation and repressor functions. J. Biol. Chem. 277: 30901-30913, 2002.
2. Maekawa, M., Yamaguchi, K., Nakamura, T., Shibukawa, R., Kodanaka, I., Ichisaka, T., Kawamura, Y., Mochizuki, H., Goshima, N., Yamanaka, S. reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature 474: 225-229, 2011.
3. Nakanishi, G., Kim, Y.-S., Nakajima, T., Jetten, A. M. Regulatory role for Kruppel-like zinc-finger protein Gli-similar 1 (Glis1) in PMA-treated and psoriatic epidermis J. Invest. Derm. 126: 49-60, 2006.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3FJ
Available
Manufacturer - Category
Primary Antibodies
Manufacturer - Isotype
Rabbit IgG
Storage Conditions
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Observed Molecular Weight
66 kDa
Clonality
Polyclonal
Application Details
Western blot, 0.25-0.5 ?g/ml/ml, Human

Immunohistochemistry(Paraffin-embedded Section), 2-5 ?g/ml/ml, Human

Immunocytochemistry/Immunofluorescence, 5 ?g/ml/ml, Human

Flow Cytometry (Fixed), 1-3 ?g/1x106 cells, Human

ELISA, 0.1-0.5 ?g/ml/ml, Human
Manufacturer - Gene Name
GLIS1
Gene Full Name
histone cluster 1 H3 family member a/b/c/d/e/f/g/h/i/j
Background
Glis1 (Glis Family Zinc Finger 1) is gene encoding a Krüppel-like protein of the same name whose locus is found on Chromosome 1p32.3. GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription.
Immunogen
E.coli-derived human GLIS1 recombinant protein (Position: M1-T620).
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purification
Immunogen affinity purified.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category
DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling, RNA Processing
Protein Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Localization
Cytoplasm.
Description
Boster Bio Anti-GLIS1 Antibody Picoband® catalog # A12698-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug/vial
Available: In stock
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