Anti TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb (Clone 11-9) (in PBS)

ELISA, IHC(f), WB

CAC

Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb 11-9 was cloned from mice immunized with diphospho-peptide [CMDSKS(p)S(p)GWGM]. 11-9 recognizes human TDP-43 phosphorylated on serines 409 and 410. 11-9 is validated for western blot, ELISA and IHC(f). This antibody is a powerful tool for biochemical and immunohistochemical analyses of TDP-43 proteinopathy.
TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases.
This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.
Cat. No. CAC-TIP-PDT-M01A Product Specifications
Product type
| primary antibody
Immunogen
| CMDSKS(p)S(p)GWGM, S(p): phosphoserine 409/410
Raised in
| mouse
Clone name
| 11-9
Isotype
| IgG1
Source
| culture supernatant
Purification
| affinity purified
Form
| liquid containing PBS
Volume
| 100 uL
Label
| unlabeled
Specificity
| phospho-TDP-43
Cross reactivity
| human
Storage
| below -20° C and below -70° C for prolonged storage; aliquot to avoid freeze/thaw cycles.
Important Information on Clone 11-9 Formulation History:
Currently shipping (since November 2022) CAC-TIP-PTD-M01A replaces discontinued CAC-TIP-PTD-M01.
-M01A (currently shipping; lot nos. 11-9-12-31-1 and newer): Affinity purified in PBS; Concentration: 1.0-1.5 mg/ml.
-M01 (lots 11-9-20 and 11-9-21): Ammonium sulfate precipitated; presented in 50% glycerol
-MO1 (prior to lot 11-9-20): provided as hybridoma culture supernatant.
Recommended dilutions:
-Western blotting 1/1000-1/3000
-Immunohistochemistry 1/3000-1/10000
-ELISA 1/1000-1/5000
-Other applications not tested
-Optimal dilutions/concentrations should be determined by end user.
References
-
-Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473

Monoclonal

phospho-TDP-43