Mouse monoclonal antibody to human laminin alpha4

Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination, laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their alpha chain, are expressed in a cell- and tissue-specific manner, and are differentially
recognized by integrins

Mouse monoclonal

Immunoaffinity-purified Laminin 411 (laminin-8 (alpha4beta1gamma1) from platelets

ELISA titer 0.02 ug/ml; WB, reduced conditions; 0.5 to 1 ug/ml; IP 0.5 mg/ml; IHC dilution 5 to 10 ug/ml

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. (2004) An endothelial laminin isoform, laminin-8 (alpha4beta1gamma1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66.

Kawataki T, Yamane T, Naganuma H, Rousselle P, Anduren I, Tryggvason K, Patarroyo M. (2007)
Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. Exp Cell Res

Q16363