Mouse monoclonal antibody to HIV-1 Nef

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Mouse monoclonal

Recombinant Nef protein of HIV-1 B subtype consensus (purified from E. coli)

ELISA 0.033 to 0.1 ug/ml; WB: 1 ug/ml



Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994. Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Fiala, A.M., R.H. Rhodes, P. Shapshak, I. Nagano, O. Martinez-Maza, A. Diagne, G. Baldwin, and M. Graves. 1996. Regulation of HIV-1 infection in astrocytes: Expression of NEF, TNF-alpha and IL-6 is enhanced in coculture of astrocytes with macrophages. J.Neurovirology, 2, No. 3: 158-166. PMID: 8799208

Koedel, U., Kohleisen B, Sporer B, Lahrtz F, Ovod V, Fontana A, Erfle V, Pfister HW. 1999. HIV type 1 Nef protein is a viral factor for leukocyte recruitment into the central nervous system. J Immunol. 1; 163(3):1237-45. PMID: 10415019

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.


Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61

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