Mouse monoclonal antibody to HIV-1 Tat

Mouse monoclonal

Recombinant Tat protein of HIV-1 HXB2 (purified from E. coli)

ELISA 0.02 to 0.05 ug/ml, WB 1 to 2 ug/ml.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn (1994) Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.

Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn (1995) Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Tähtinen, M., Ranki, A., Valle, S.L., Ovod, V., K. Krohn (1997) B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51: 480-7.

Henderson WW, Ruhl R, Lewis P, Bentley M, Nelson JA and Moses AV. (2004) Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78: 4408-20.
Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren (2001) Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.