Mouse monoclonal antibody to Hev b1 (Rubber elongation factor, REF)

Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02.

Mouse monoclonal

Hev b1-MBP fusion protein expressed and purified from E. coli.

ELISA 1:32 000. Reacts as binding antibody in capture ELISA with Hev b1 detection monoclonal antibody A3-700-100. WB 1:2000 to 1:4000
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ASTM D7427 - 08e1 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived from Latex.

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Peixinho C, Tavares-Ratado P, Tomas MR, Taborda-Barata L, Tomaz CT. (2008) Latex allergy: new insights to explain different sensitization profiles in different risk groups. Br J Dermatol. 159(1):132-6.
Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C, Meyer DM. (2007) The immuno cross-reactivity of gutta percha points. Dent Mater. 23(3):380-4

P15252