Bcl-2 IHC Antibody

Description Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14; 18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line. Catalog Number IW-MA1004 Quantity 9 ml Host Mouse Clone BL-2 Isotype Mouse IgG1 Immunogen Synthetic peptide corresponding to residues 41-54pf the bcl-2 protein, conjugated to thyroglobulin. Purity Purified by the goat anti-mouse IgG affinity chromatography Conjugate Unconjugated Species Reactivity Human. Not tested in other species. Positive Control Human tonsil Cellular Localization Membrane/cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Fr: Formalin fixed or acetone fixed frozen sections. ICC: Suitable. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Ngan, B.-Y.; Chen-Levy, Z.; Weiss, L. M.; Warnke, R. A.; Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t(14; 18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988. 2. Hockenbery, D.; Nunez, G.; Milliman, C.; Schreiber, R. D.; Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990