beta Amyloid Protein IHC Antibody

Description Beta amyloid protein is 4.2-kD polypeptide. Glenner and Wong(1984) purified a protein derived from the twisted beta-pleated sheet fibrils present in cerebrovascular amyloidoses and in the amyloid plaques associated with Alzheimer disease. The proteins from both disorders have an identical 28-amino acid sequence. memory deficits in middle-aged Tg2576 mice are caused by the extracellular accumulation of a 56-kDa soluble amyloid-beta assembly. Amyloid beta protein(Abeta) deposition in the brain is a hallmark of Alzheimer's disease(AD). Catalog Number IW-MA1109 Quantity 9 ml Host Mouse Clone AP-5 Isotype Mouse IgG1 Immunogen Synthetic beta-amyloid peptide (1-40), conjugated to KLH. Purity Purified by the goat anti-mouse IgG affinity chromatography Conjugate Unconjugated Species Reactivity Human, mouse. Not tested in other species. Positive Control Alzheimer's brain tissue Cellular Localization Extracellular senile plaque and neurofibrillary tangles Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Formic acid pretreatment or heat induced epitope retrieval (HIER) is required on formalin fixed paraffin embedded tissue sections. IHC-Fr: Not tested. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Lesne, S.; Koh, M. T.; Kotilinek, L.; Kayed, R.; Glabe, C. G.; Yang, A.; Gallagher, M.; Ashe, K. H. : A specific amyloid-beta protein assembly in the brain impairs memory. Nature 440: 352-357, 2006. 2. Lorenzo, A.; Yuan, M.; Zhang, Z.; Paganetti, P. A.; Sturchler-Pierrat, C.; Staufenbiel, M.; Mautino, J.; Vigo, F. S.; Sommer, B.; Yankner, B. A. : Amyloid beta interacts with the amyloid precursor protein: a potential toxic mechanism in Alzheimer's disease. Nature Neurosci. 3: 460-464, 2000.