ABI-1 IHC Antibody

Description ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL. Catalog Number IW-PA1001 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human Abi-1, different from the related rat and mouse sequence by single amino acid. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat. Not tested in other species. Positive Control Rat brain and spleen, human intestine, Hela cell Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Proteinase K digestion is required on formalin fixed paraffin sections. IHC-Fr: Formalin or acetone fixed frozen section. ICC: Acetone fixed cells. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1.Tani, K.; Sato, S.; Sukezane, T.; Kojima, H.; Hirose, H.; Hanafusa, H.; Shishido, T. : Abl interactor 1 promotes tyrosine 296 phosphorylation of mammalian Enabled (Mena) by c-Abl kinase. J. Biol. Chem. 278: 21685-21692, 2003. 2. Taki, T.; Shibuya, N.; Taniwaki, M.; Hanada, R.; Morishita, K.; Bessho, F.; Yanagisawa, M.; Hayashi, Y. : ABI-1, a human homolog to mouse Abl-interactor 1, fuses the MLL gene in acute myeloid leukemia with t(10; 11)(p11.2; q23). Blood 92: 1125-1130, 1998.