TNF-alpha IHC Antibody

Description TNFalpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. Catalog Number IW-PA1079 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human TNFalpha, different from the related mouse sequence by five amino acids, and rat sequence by seven amino acids. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat.Not tested in other species. Positive Control Human mammary cancer, colon cancer, rat brain Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (Cat# IW-1100) is recommended. IHC-Fr: Not tested. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B. Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985. 2. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984.