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Anti-Human/Mouse/Rat Activin A - Biotin - 50 µg

Anti-Human/Mouse/Rat Activin A - Biotin - 50 µg

Item no.	LEIN-A397-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Caprine
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Human/Mouse/Rat Activin A this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human/Mouse/Rat Activin A is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:A213.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines
Manufacturer - Targets	Activin A
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Activin A is a member of the TGF beta super family. It was originally identified and isolated because it stimulates the release of follicle-stimulating hormone (FSH). Activins are structurally related to inhibins, they share common beta subunits, but they exhibit opposite biological activities. Activins are homodimers of beta subunits and inhibins are heterodimers of an alpha subunit and a beta subunit. Since the initial discovery of Activin A, it has been shown to regulate cell differentiation, nerve cell survival, bone growth promotion, cell proliferation, and apoptosis. Activin A expression has been detected in prostate cancer, colon cancer and breast cancer. Because Activin A is able to inhibit cell proliferation by modulating the Rb pathway, it is thought to inhibit cancer development. This may not be the only mechanism by which activin may play a preventative role in cancer development. Activin has also been shown to increase the expression of a protein whose decreased expression is correlated with increased metastatic capacity. In addition, activin has an inhibitory effect on endothelial cell growth and can thereby block angiogenesis, preventing tumor growth and metastasis. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (βA - βA), activin B (βB - βB), activin AB (βA - βB), inhibin A (α - βA) and inhibin B (α - βB). At present, little is known about the contribution of the other β subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human βA subunit is 100% identical to mouse βA, while the human and mouse α subunits share approximately 80% identity. Similarly to other TGF-β family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling.
Background	Activin A is a member of the TGF beta super family. It was originally identified and isolated because it stimulates the release of follicle-stimulating hormone (FSH). Activins are structurally related to inhibins, they share common beta subunits, but they exhibit opposite biological activities. Activins are homodimers of beta subunits and inhibins are heterodimers of an alpha subunit and a beta subunit. Since the initial discovery of Activin A, it has been shown to regulate cell differentiation, nerve cell survival, bone growth promotion, cell proliferation, and apoptosis. Activin A expression has been detected in prostate cancer, colon cancer and breast cancer. Because Activin A is able to inhibit cell proliferation by modulating the Rb pathway, it is thought to inhibit cancer development. This may not be the only mechanism by which activin may play a preventative role in cancer development. Activin has also been shown to increase the expression of a protein whose decreased expression is correlated with increased metastatic capacity. In addition, activin has an inhibitory effect on endothelial cell growth and can thereby block angiogenesis, preventing tumor growth and metastasis. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (βA - βA), activin B (βB - βB), activin AB (βA - βB), inhibin A (α - βA) and inhibin B (α - βB). At present, little is known about the contribution of the other β subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human βA subunit is 100% identical to mouse βA, while the human and mouse α subunits share approximately 80% identity. Similarly to other TGF-β family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling.
PubMed	Activin A
Manufacturer - Specificity	Goat Anti-Human Activin A recognizes Human Activin A. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2828145
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen	Purified Recombinant Human/Mouse/Rat Activin A (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Activin A, Human, Anti-Human/Mouse/Rat Activin A-Biotin

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-A397-50ug-safety-datasheet.pdf