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Anti-Human CD30 Ligand-Biotin - 50 µg

Anti-Human CD30 Ligand-Biotin - 50 µg

Item no.	LEIN-C1488-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Croager, EJ. et al. (1997) Biochim. Biophys. Acta. 1353:231 2. Gruss, HJ. et al. (1994) Blood 83:1521 3. Wendtner, CM. et al. (1995) Cancer Res. 55:4157 4. Fischer, M. et al. (2006) J. Clin. Invest. 116:2748 5. Blazar, BR. et al. (2004) J. Immunol. 173:2933
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Human CD30 Ligand this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CD30 Ligand is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines
Manufacturer - Targets	CD30 Ligand
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.
Background	The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.
PubMed	CD30 Ligand
Manufacturer - Specificity	Goat Anti-Human CD30 Ligand, also known as CD153 antigen recognizes Human CD30 Ligand. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2828554
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen	NS0-Derived Recombinant Human CD30 Ligand Extracellular Domain (Accession # P32971)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, CD30 Ligand, Human TNFSF8, CD153, Human CD153, Anti-Human CD30 Ligand/TNFSF8-Biotin, CD153 antigen, CD153, CD30 antigen ligand, Human CD30 Ligand, CD30L, CD30-L, CD30LCD30 ligand, CD30LGMGC138144, TNFSF8, tumor necrosis factor (ligand) superfamily member 8, tumor necrosis factor ligand superfamily member 8

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1488-50ug-safety-datasheet.pdf