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Anti-Mouse CD3ε (Clone 145-2C11) - Purified - 500 µg

Anti-Mouse CD3ε (Clone 145-2C11) - Purified - 500 µg

Item no.	LEIN-C1757-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot Immunocytochemistry Primary Antibodies Immunochemistry Functional Assays
Type	Antibody Monoclonal
Applications	WB, FC, IP, IHC, ICC, FA, other
Clone	145-2C11
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
NCBI	12501
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Act, B, FA, FC, ICC, IHC FF, IP, PhenoCycler®, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD3ε
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This purified antibody is stable when stored at 2-8°C. Do not freeze.
Product Description	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Background	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Other Applications Reported In Literature	IHC (Frozen) The suggested concentration for this 145-2C11 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. IP Act B WB ICC
PubMed	CD3ε
Ligand/Receptor	Peptide antigen/MHC-complex
Manufacturer - Research Area	Immunology
Manufacturer - Specificity	Clone 145-2C11 recognizes an epitope on mouse CD3ε.
RRID	AB_2828823
Concentration	0.5 mg/ml
Formulation	This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen	H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, CD3ε, CD3, T3, 145-2C11, CD3ε, Mouse, Anti-Mouse CD3ε Purified, Flow Cytometry, Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Western Blotting, Immunology

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1757-500ug-safety-datasheet.pdf