Anti-Mouse ICOSL (CD275) (Clone HK5.3) - Biotin

Primary Monoclonal Antibodies>Immune Checkpoints|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers

USA

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

B7-H2, also known as ICOSL and B7RP-1, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. B7-H2 is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function (1). ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway (2). The structural features of B7-H2 are crucial for its costimulatory function. B7-H2 constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein (3).

Additional reported applications (for the relevant formats) include:
Blocking

ICOS

Rat Anti-Mouse B7-H2 (CD275) (Clone HK5.3) recognizes an epitope on Mouse CD275. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

0.5 mg/ml

This monoclonal antibody was purified by affinity chromatography, and biotinylated under optimal conditions.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.