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Anti-Human CD3 (Clone HIT3a) - Purified in vivo GOLD™ Functional Grade

Anti-Human CD3 (Clone HIT3a) - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-C2446-0.5mg
Manufacturer	Leinco Technologies
Amount	0.5 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IV
Clone	HIT3a
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
Citations	1. Meuer, S. C. et al. (1983) Nature 303:808 2. van Dongen, J. J. M. et al. (1988) Blood 71:603 3. Beverly, P. C. L. et al. (1981) Eur. J. Immunol. 11:329 4. Leukocyte Typing: 3rd Workshop: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
NCBI	916
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this HIT3a antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD3
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Background	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
PubMed	CD3
Ligand/Receptor	Peptide antigen bound to MHC
Manufacturer - Specificity	Clone HIT3a recognizes an epitope on human CD3.
RRID	AB_2829543
Concentration	1.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Endotoxin Level	≤ 1.0 EU/mg as determined by the LAL method
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C2446-0.5mg-safety-datasheet.pdf