« Back
Home /
Anti-Human IgM µ-Chain Specific (Clone HB57) – Purified in vivo GOLD™ Functional Grade - 0.25 mg

Anti-Human IgM µ-Chain Specific (Clone HB57) – Purified in vivo GOLD™ Functional Grade - 0.25 mg

Item no.	LEIN-I-1202-0.25mg
Manufacturer	Leinco Technologies
Amount	0.25 mg
Category	In Vivo Antibodies Flow Cytometry ELISA Primary Antibodies Immunostaining
Type	Antibody Monoclonal
Applications	FC, ELISA, IV
Clone	HB57
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
Citations	1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies
Manufacturer - Targets	Human Immunoglobulin, IgM µ-Chain Specific
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Cross Reactivity	Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: < 0.2% Human IgA: < 0.1% Human IgE: < 0.1% There is no detectable binding to bovine, goat, horse sheep IgG. NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201)
PubMed	IgM µ-Chain Specific
Manufacturer - Specificity	Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 10⁸ M^-1).¹
RRID	AB_2893816
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Surface IgM is expressed on B-lymphocytes.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, IgM µ-Chain Specific, HB57, Human IgM gamma chain specific, Anti-Human IgM µ-Chain Specific Purified, Clone HB57, ELISA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

LEIN-I-1202-0.25mg-safety-datasheet.pdf