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Anti-Human ICOS - Biotin - 50 µg

Anti-Human ICOS - Biotin - 50 µg

Item no.	LEIN-I-754-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Category	Oncology Antibodies By Cell Type Hematology Lymphoma Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Flavell, RA. et al. (2001) Nature 409: 97 2. Bachmann, MF. et al. (2000) J Exp Med. 192: 53 3. Kroczek, RA. et al. (1999) Nature 397: 263
NCBI	29851
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Human ICOS this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human ICOS is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-551.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines\|Primary Monoclonal Antibodies>Immune Checkpoints
Manufacturer - Targets	ICOS
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Inducible co-stimulator (ICOS) is an inducible homodimeric costimulator protein that is structurally and functionally related to CD28. It is a third member of the CD28/CTLA4 family and it is expressed on activated T cells.1ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses.2 ICOS is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases.3
Background	Inducible co-stimulator (ICOS) is an inducible homodimeric costimulator protein that is structurally and functionally related to CD28. It is a third member of the CD28/CTLA4 family and it is expressed on activated T cells.¹ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses.² ICOS is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases.³
PubMed	ICOS
Manufacturer - Specificity	Goat Anti-Human Inducible Costimulator (ICOS) recognizes Human ICOS. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2830875
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen	Purified Recombinant Human ICOS (Accession # Q9Y6W8)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Inducible Co-Stimulator, AILIM, ICOS, Human ICOS, Anti-Human ICOS-Biotin, Activation-inducible lymphocyte immunomediatory molecule, AILIM, AILIMCD278, CD278 antigen, CD278, CRP-1, CVID1, ICOS, inducible costimulator, inducible T-cell costimulator, inducible T-cell co-stimulator, MGC39850

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-754-50ug-safety-datasheet.pdf