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Anti-Mouse IL-21 - Biotin

Anti-Mouse IL-21 - Biotin

Item no.	LEIN-I-787-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, ELISA
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
Citations	1. Foster, D. et al. (2000) Nature 408: 57 2. Clegg, CH. et al. (2002) J. Leukoc. Biol. 72: 856 3. Leonard, WJ.et al. (2002) Science 298: 1630 4. Joannopoulos, K.et al. (2003) J. Immunol. 171: 608 5. Lipsky, PE. et al. (2008) Ann Rheum. Dis. 3: 83
NCBI	60505
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Mouse IL-21 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-21 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Mouse IL‑21 Antibody. Standard: Recombinant Mouse IL-21 Protein.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines
Manufacturer - Targets	IL-21
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Interleukin 21 (IL-21) is a T cell-derived pleiotropic cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells.1, 2 This cytokine induces cell division/proliferation in its target cells. IL-21 elicits its effects on immune cells by interacting with a cell surface receptor known as the interleukin 21 receptor, IL-21R, which is expressed in bone marrow cells and various lymphocytes. IL-21 is closely related to IL-2 and IL-153 and is a key factor in the transition between innate and adaptive immune responses.4 The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterised by overproduction of pathogenic autoantibodies.5
Background	Interleukin 21 (IL-21) is a T cell-derived pleiotropic cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells.^{1, 2} This cytokine induces cell division/proliferation in its target cells. IL-21 elicits its effects on immune cells by interacting with a cell surface receptor known as the interleukin 21 receptor, IL-21R, which is expressed in bone marrow cells and various lymphocytes. IL-21 is closely related to IL-2 and IL-15³ and is a key factor in the transition between innate and adaptive immune responses.⁴ The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterised by overproduction of pathogenic autoantibodies.⁵
PubMed	IL-21
Manufacturer - Specificity	Goat Anti-Mouse Interleukin 21 (IL-21) recognizes Mouse IL-21. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2830908
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen	E. coli-Derived Recombinant Mouse Interleukin-21 (Accession # Q9ES17)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-787-50ug-safety-datasheet.pdf