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Anti-Human HER-2 (Trastuzumab) - HRP

Anti-Human HER-2 (Trastuzumab) - HRP

Item no.	LEIN-LT1502-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	FC, ELISA
Clone	4D5-8
Specific against	Human (Homo sapiens)
Host	Human
Citations	1. Fendly, B. et al. (1990) Cancer Research 50: 1550-1558. 2. McBride, H. et al. (2019) Pharm Res. 36(12): 177. 3. Zielinski, C. et al. (1997) Int. J. Cancer 73: 875–879 4. Valone, FH. et al. (1995) J. Clin. Oncology 13 (9): 2281-92. 5. Hynes, NE. et al. (1993) Br J Cancer. 68(6): 1140–1145.
NCBI	2064
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for Trastuzumab biosimilar antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Recombinant Antibodies>Biosimilar Recombinant Antibodies
Manufacturer - Targets	HER-2/neu
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8°C. Do not freeze.
Product Description	Trastuzumab is a monoclonal antibody targeting HER2, a 185 kDa transmembrane glycoprotein that contains an extracellular domain and intracellular tyrosine kinase activity. When it is functioning normally, the HER2 pathway supports cell growth and division. On the other hand, the over expression of HER2 propels cell growth beyond its typical range. This overexpression is associated with some cancers, namely breast and stomach, in which the HER2 protein can be expressed up to 100 times more than in typical cells. Trastuzumab induces an immune-mediated response that triggers the internalization and downregulation of HER2 making it an excellent target for immunotherapy. Several clinical studies are under way which show that anti-HER-2/neu antibodies inhibit the growth and proliferation of these tumor cells In vitro as well as In vivo.
Background	Trastuzumab is a monoclonal antibody targeting HER2, a 185 kDa transmembrane glycoprotein that contains an extracellular domain and intracellular tyrosine kinase activity. When it is functioning normally, the HER2 pathway supports cell growth and division. On the other hand, the over expression of HER2 propels cell growth beyond its typical range. This overexpression is associated with some cancers, namely breast and stomach, in which the HER2 protein can be expressed up to 100 times more than in typical cells. Trastuzumab induces an immune-mediated response that triggers the internalization and downregulation of HER2 making it an excellent target for immunotherapy. Several clinical studies are under way which show that anti-HER-2/neu antibodies inhibit the growth and proliferation of these tumor cells In vitro as well as In vivo.
Additional Reported Applications For Relevant Conjugates	CyTOF® IHC
PubMed	HER-2/neu
Manufacturer - Expression host	HEK-293
Manufacturer - Specificity	This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. Clone 4D5-8 recognizes human erbB-2. This product is for research use only.
FC Effector Activity	Active
RRID	AB_2893912
Concentration	0.5 mg/ml
Formulation	This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase).
Antigen Distribution	Ubiquitous expression with highest expression levels found in the kidney, skin, esophagus, and small intestine.
Immunogen	Human epidermoid carcinoma cells (A431) over-expressing EGFR.
Regulatory Status	Research Use Only (RUO). Non-Therapeutic.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-LT1502-100ug-safety-datasheet.pdf