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Anti-Mouse TCR β chain (TCR) - Purified - 500 µg

Anti-Mouse TCR β chain (TCR) - Purified - 500 µg

Item no.	LEIN-T648-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC
Clone	H57-597
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Citations	1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7
NCBI	21577
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Costim, Depletion, FC, IHC FF, IHC FFPE, PhenoCycler®
Manufacturer - Category	Mouse T-Cell Receptor Complex\|PhenoCycler®
Manufacturer - Targets	TCR beta
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Product Description	TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3, 4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5, 6.
Background	TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells¹. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond². TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling^{3, 4}. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival^{5, 6}.
Function	Antigen recognition, T cell activation
Additional Reported Applications For Relevant Conjugates	CODEX®
Other Applications Reported In Literature	Costim Depletion IP IHC FF IHC FFPE
PubMed	TCR beta
Ligand/Receptor	Peptide bound-MHC class I and II
Manufacturer - Specificity	Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
RRID	AB_2893101
Concentration	0.5 mg/ml
Formulation	This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.
Immunogen	Affinity purified TCR from mouse DO-11.10 cells
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, TCR beta, H57-597, TCR β, Mouse, Anti-Mouse β (TCR) - Purified, Costimulation, Depletion, TCR

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T648-500ug-safety-datasheet.pdf