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Anti-Mouse VEGF - Biotin

Anti-Mouse VEGF - Biotin

Item no.	LEIN-V106-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, ELISA
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
Citations	1. Folkman, J. et al. (2008) FASEB J. 22:3728 2. Goodsell, DS. et al. (2002) The Oncologist 7:569 3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13:130 4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312:549 5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28:488 6. Ellis, LM. et al. (2005) J Clin Oncol. 23:1011
NCBI	22339
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Mouse VEGF this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse VEGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with as the capture antibody at 0.2-0.8 µg/ml.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines
Manufacturer - Targets	VEGF
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3, 4, 5, 6
Background	Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine¹ is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.² VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.^{3, 4, 5, 6}
PubMed	VEGF
Manufacturer - Specificity	Goat Anti-Mouse Vascular Endothelial Cell Growth Factor (VEGF) recognizes Mouse VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2832133
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen	Purified Recombinant Mouse VEGF (Accession # AAA40547)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-V106-50ug-safety-datasheet.pdf